NIA Mouse ES Cell Bank
NIA Mouse ES Cell Bank
ES Cell Bank
Background
Strategy
Progress
Methods
Cell lines
Microarrays
ChIP-seq
Protein complexes
Publications
Download
FAQ
Lab Home page
Software/Database
Array Analysis
Gene Index
CisView
CisFinder
mRNA Decay Rate
Protein Interactions
Project objectives:
  • Generate 300 plasmid constructs that can express FLAG-tagged Open Reading Frames (ORFs) encoding mostly transcription factors in Tetracycline-inducible manner
  • Generate 300 mouse ES cell lines, in each of which one of 300 TFs tagged with a FLAG moiety is inserted into a ubiquitously controllable tetracycline-repressible locus
  • Test the quality of the ES cell lines and make them available as resources for the scientific community
  • Quantify the effect of induced TFs on the expression of other genes with whole-genome microarrays, and use these data to reconstruct gene regulatory networks
  • Use FLAG-tag immuno-precipitation (IP) of transgenic TFs for the analysis of TF-binding sites (ChIP-seq) and protein complexes associated with the TF (Mass Spectrometry)
  • Explore other applications, such as the analysis for the subcellular localization of TFs by FLAG immuno-histochemistry, TF knockdown by shRNAs, differentiation of ES cells into specific cell types, and transgenic mouse models
How to use this web site
Use the menu bar (at the left) to access the overview of the project, methods, quality control summary for 54 ES cell lines that are ready for distribution, microarray data, information on the use of ES Cell Bank for ChIP-seq, analysis of protein complexes, and development of transgenic mice. The FAQ section explains how to obtain the transgenic ES cell lines.
Funding
This research was supported entirely by the Intramural Research Program of the NIH, National Institute on Aging.

 

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